Novel Liquid Chromatography–Tandem Mass Spectrometry Method for Analysis of 102 Pesticides and Five Mycotoxins Regulated by the State of Colorado in Dried Hemp

A novel liquid chromatography–tandem mass spectrometry (LC–MS/MS) method with a dual electrospray ionization (ESI) and atmospheric pressure chemical (APCI) source was developed for analyzing 102 pesticides five mycotoxins that are regulated by the state of Colorado in hemp. The limit quantitation (LOQ) met action requirements these analytes hemp matrix. For this study, 88 out all were analyzed using LC–MS/MS an ESI source, whereas remaining 14 determined APCI source. simple, fast, green, cheap acetonitrile solvent extraction used to extract from matrix good efficiency range 80–120%. is challenging causes effects such as ion suppression or enhancement. We optimized LC added 30 internal standards reduce compensate obtain accuracy 70–120%. mechanism nonpolar (normally gas [GC–MS/MS]) elucidated.